TaKaRa Taq Hot Start Version GMP grade DNA polymerase在严格的标准下制造，以确保高质量和一致性，符合GMP规定和指南的设施。GMP级别为热启动聚合酶带来了更高水平的可信度和一致性，特别适用于苛刻的研究应用，包括多重PCR。 此GMP级试剂提供：
· 严格的规格
· 卓越的性能
· 可定制包装
所有的Hot-start型Takara Taq聚合酶是抗Taq单克隆抗体和TaKaRa Taq 的混合制品，适用于Hot Start PCR。Hot Start PCR可以提高特异性，降低背景，可在室温下配制反应液。因Hot Start PCR可防止非特异性扩增，高特异性是实验成功的关键，因此是多重PCR的理想选择。抗Taq单克隆抗体在PCR反应最初的DNA变性步骤已变性，因此在常规PCR反应条件下即可使用。
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Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711,6,127,155 and claims outside the US corresponding to expired US Patent No.5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claims, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration,is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
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■ 保存
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-20℃。避免反复冻融。
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■ 特点
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Test Items
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Specifications
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Results
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Purity (microfluidic chip analysis)
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>98%
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Passed
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Ribonucleases
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No observable degradation
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Passed
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Endonucleases
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No observable degradation
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Passed
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Exonucleases
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No observable degradation
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Passed
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Nicking activities
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No observable relaxation of supercoiled DNA
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Passed
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Residual E. coli gDNA
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<10 copies/1.25 U
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Passed
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Hot-start activity
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Confirmed
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Passed
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PCR performance
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8-kb fragment was amplified
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Passed
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Sensitivity (λ DNA)
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0.5-kb fragment was amplified from 10 pg of λ DNA
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Passed
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Sensitivity (human gDNA)
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0.1-kb fragment was amplified from 30 pg of human
genomic DNA
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Passed
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■ 应用
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· Hot-start PCR
· Multiplex PCR
· qPCR
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