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In-Fusion克隆工具

2018-08-29文章来源：Takarabiomed

In-Fusion Cloning tools

Design your primers

NEW TOOL!

Our NEW In-Fusion Cloning Primer Design Tool allows for single- or multiple-insert cloning, accommodates vector linearization by inverse PCR or restriction digest, and enables site-directed mutagenesis. Simply input the DNA sequences of your vector and insert(s), along with your linearization method to generate primers for your next cloning experiment. Easily switch to the mutagenesis option to generate primers for all of your insertion, replacement, and deletion projects.

Launch »

Molar ratio calculator for In-Fusion Cloning

Calculate the optimal amounts of vector and insert for an In-Fusion Cloning reaction. Just enter the sizes of both vector and insert(s), in base pairs.

Simulate your In-Fusion Cloning construct

SnapGene software allows easy planning and visualization of molecular biology procedures, including In-Fusion Cloning.

Cloning shouldn't be work, it should just work ...

With In-Fusion HD Cloning Plus kits, you will be able to perform directional, seamless cloning of any PCR fragment—or multiple fragments—into any linearized vector in a single 15-minute reaction, with >95% cloning efficiency. In-Fusion HD Cloning Plus is a complete system that includes all of the reagents you need for your cloning experiments: PCR enzyme, competent cells, and a kit for PCR cleanup or gel extraction. We have optimized the reagents to work together to give you the right clone the first time.

Learn about the liquid kits Learn about the lyophilized kits